Macaque Corpus Callosum G-Ratio Analysis

This book presents a morphometric analysis of myelinated axons across 8 regions of the macaque corpus callosum (CC). The goal is to characterize the relationship between axon diameter, fiber diameter, and g-ratio across CC regions, and to determine whether the g-ratio is constant or regionally variable.

Data¶

• Species: Macaque

• Structure: Corpus callosum, 8 regions (Region 1 = genu through Region 8 = splenium)

• Images per region: 3 histology image fields of view

• Pixel size: 0.009144 um

• Image dimensions: 2300 x 3040 pixels

Pipeline¶

1. Segmentation: Axon and myelin masks were generated using AxonDeepSeg (v5.4.0), then manually corrected.

2. Morphometrics: Per-axon measurements (diameter, area, g-ratio, myelin thickness, etc.) were computed using axondeepseg_morphometrics.

3. Filtering: Axons touching the image border, unmyelinated axons, and invalid segmentations were removed.

4. Analysis: Linear regression of axon diameter vs fiber diameter and g-ratio vs fiber diameter.

Contents¶

• Macaque Corpus Callosum G-Ratio Analysis
• Morphometrics Analysis
• Morphometrics Analysis (ADS 2015 — No QC)
• Morphometrics Analysis (ADS 2026 — Full QC)
• Quality Control
  • Quality Control
  • Region 1
  • Region 2
  • Region 3
  • Region 4
  • Region 5
  • Region 6
  • Region 7
  • Region 8